环境高温诱导巴马香猪睾丸细胞凋亡并激活Nrf2/HO-1抗氧化信号通路

doi:10.11843/j.issn.0366-6964.2018.06.007

Abstract

Abstract:

The objective of this study was to determine the heat sensitivity index for Bama miniature pigs by investigating the effect of ambient high temperature on scrotal temperature distributions and testicular protein expressions related to the antioxidative capacity and cellular apoptosis in the pigs. Twelve 7-month-old mature Bama miniature pigs were selected and randomly allotted to a thermal neutral(TN)group and a heat-treated(HT)group. The HT group was exposed to high ambient temperature, which was changed from 25 to 40℃ and back to 25℃ during each 24 h period for 8 d. At the end of the experiment, testes were collected and measured for the protein expression of nuclear factor erythroid 2-related factor 2(Nrf2), heme oxygenase 1(HO-1)and cleaved caspase 3 (c-caspase 3)using immunohistochemical staining. Results showed that, the HT group pigs presented a significant increase in scrotal surface temperature(P<0.05), which was 3.7℃ higher than the TN group pigs. In the HT group, the testicular protein expression of Nrf2 and HO-1 increased significantly in both the testicular interstitial cells and the germ line cells of seminiferous epithelium(P<0.05). Moreover, prominent nuclear accumulation of Nrf2 protein was observed in the testicular interstitial cells. In the HT group, there was an increased percentage of the c-caspase 3-positive nucleuses in the testicular interstitial cells and germ line cells of seminiferous epithelium(P<0.05), respectively. The percentage of the c-caspase 3-positive nucleuses in the testicular interstitial cells increased more sharply than that in the germ line cells of seminiferous epithelium. In conclusion, the capacity of scrotal thermal regulation was overwhelmed in the 40℃ ambient temperature, which increased the testicular temperature and caused the testicular cell apoptosis. High ambient temperature also activated Nrf2 signal pathway and increased HO-1 expression, indicating that the testicular Nrf2/HO-1 signal pathway might be a potential target for new strategies to protect semen quality from high ambient temperature-induced damage in boars.

CLC Number:

S828.8

LI Yan-sen, LI Zhao-jian, JIA Xiao-xiao, LI Chun-mei. Ambient High Temperature Induced Cellular Apoptosis and Nrf2/HO-1 Signal Pathway in Testes of Bama Miniature Pigs[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(6): 1154-1162.

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Ambient High Temperature Induced Cellular Apoptosis and Nrf2/HO-1 Signal Pathway in Testes of Bama Miniature Pigs

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